ARG42298

anti-CD120a / TNFR1 antibody [H398] (PE)

anti-CD120a / TNFR1 antibody [H398] (PE) for Flow cytometry and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [H398] recognizes CD120a / TNFR1
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody H398 recognizes the extracellular domain of CD120a, a 55 kDa receptor for tumor necrosis factor. The antibody blocks biological activity of both natural and recombinant human TNF alpha and TNF beta.
Host Mouse
Clonality Monoclonal
Clone H398
Isotype IgG2a
Target Name CD120a / TNFR1
Antigen Species Human
Immunogen Recombinant full length Human CD120a.
Conjugation PE
Alternate Names TNF-R; p60; TNFAR; CD antigen CD120a; TNFR55; TBP1; TNF-RI; TNFR1-d2; Tumor necrosis factor receptor superfamily member 1A; FPF; TNFR60; CD120a; TNFR1; p55; TNF-R55; TNF-R-I; MS5; TNFR-I; Tumor necrosis factor receptor 1; TBPI; Tumor necrosis factor receptor type I; TNF-R1; p55-R

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7132 Human TNFRSF1A

Swiss-port # P19438 Human Tumor necrosis factor receptor superfamily member 1A

Gene Symbol TNFRSF1A
Gene Full Name tumor necrosis factor receptor superfamily, member 1A
Background This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]
Function Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Secreted. Note=A secreted form is produced through proteolytic processing. Isoform 4: Secreted. Note=Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. [UniProt]
Calculated MW 50 kDa
PTM The soluble form is produced from the membrane form by proteolytic processing. [UniProt]