ARG65410

anti-CD138 / Syndecan 1 antibody [B-A38] (azide free)

anti-CD138 / Syndecan 1 antibody [B-A38] (azide free) for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Cancer antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication1

Overview

Product Description Azide free Mouse Monoclonal antibody [B-A38] recognizes CD138 / Syndecan 1
Tested Reactivity Hu, Ms
Predict Reactivity Gpig, Rat
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P
Specificity The antibody BA38 recognizes CD138 (syndecan 1), a heparan sulfate proteoglycan protein expressed mainly in the epidermis and plasma cells, but also in growth factorstimulated lymphocytes. The MWs of the glycosylated CD138 were variety, and MW of the non-glycosylated core protein (dimmer form) was around 60-80 kda.
Host Mouse
Clonality Monoclonal
Clone B-A38
Isotype IgG1
Target Name CD138 / Syndecan 1
Antigen Species Human
Immunogen U266 human peripheral blood myeloma cell line
Conjugation Un-conjugated
Alternate Names CD antigen CD138; syndecan; CD138; SDC; Syndecan-1; SYND1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS3-5 μg/ml
ICC/IF1:10 - 1:500
IHC-Fr1:50 - 1:500
IHC-P1:50 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20969 Mouse SDC1

GeneID: 6382 Human SDC1

Swiss-port # P18827 Human Syndecan-1

Swiss-port # P18828 Mouse Syndecan-1

Gene Symbol SDC1
Gene Full Name syndecan 1
Background CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
Function Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 32 kDa
PTM Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Specific References

Cell surface-expressed Ro52/IgG/HLA-DR complex is targeted by autoantibodies in patients with inflammatory myopathies

IHC-P / Human

Noriko Arase et al.
J Autoimmun.,  (2022)

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