ARG62727

anti-CD14 antibody [B-A8]

anti-CD14 antibody [B-A8] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody

Overview

Product Description

Mouse Monoclonal antibody [B-A8] recognizes CD14

Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P
Specificity The clone B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
Host Mouse
Clonality Monoclonal
Clone B-A8
Isotype IgG1
Target Name CD14
Antigen Species Human
Immunogen Human monocytes
Conjugation Un-conjugated
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 µg/ml
ICC/IF1:50 - 1:100
IHC-P2 - 10 µg/ml
Application Note ELISA: The clone B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (ARG62725).
IHC-P: Heat retrieval of antigen is recommeded. Staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: PML brain section

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Calculated MW 40 kDa
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Clone References

Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.

FACS / Human

Paupert J et al.
Cell Cycle.,  (2008)

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CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.

Game DS et al.
J Am Soc Nephrol.,  (2003)

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Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection.

ICC/IF / Human

Harvey HA et al.
Infect Immun.,  (2002)

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