ARG53765

anti-CD14 antibody [MEM-18] (APC)

anti-CD14 antibody [MEM-18] (APC) for Flow cytometry and Human,Primates

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [MEM-18] recognizes CD14
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
HLDA III; WS Code M 253
HLDA IV; WS Code M 314
HLDA V; WS Code M MA087
HLDA VI; WS Code M MA95
Host Mouse
Clonality Monoclonal
Clone MEM-18
Isotype IgG1
Target Name CD14
Antigen Species Human
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Conjugation APC
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Calculated MW 40 kDa
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Clone References

Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells.

Suzuki K et al.
Int Immunol.,  (2011)

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Soluble CD14 from urine copurifies with a potent inducer of cytokines.

Sundan A et al.
Eur J Immunol.,  (1994)

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Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.

Bazil V et al.
Eur J Immunol.,  (1986)

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Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.

Bazil V et al.
Mol Immunol.,  (1989)

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