ARG65491

anti-CD14 antibody [MEM-18] (low endotoxin)

anti-CD14 antibody [MEM-18] (low endotoxin) for ELISA,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [MEM-18] recognizes CD14
Tested Reactivity Hu, NHuPrm
Tested Application ELISA, FACS, FuncSt, IP, WB
Specificity The clone MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
HLDA III; WS Code M 253
HLDA IV; WS Code M 314
HLDA V; WS Code M MA087
HLDA VI; WS Code M MA95
Host Mouse
Clonality Monoclonal
Clone MEM-18
Isotype IgG1
Target Name CD14
Antigen Species Human
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Conjugation Un-conjugated
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS4 µg/ml
FuncStAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
ELISA: The clone MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with clone B-A8 (ARG62727).
Functional studies: The clone MEM-18 completely blocks binding of fluorescein (FITC) - labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Calculated MW 40 kDa
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Clone References

Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells.

Suzuki K et al.
Int Immunol.,  (2011)

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Soluble CD14 from urine copurifies with a potent inducer of cytokines.

Sundan A et al.
Eur J Immunol.,  (1994)

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Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.

Bazil V et al.
Eur J Immunol.,  (1986)

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Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.

Bazil V et al.
Mol Immunol.,  (1989)

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