ARG23485

anti-CD14 antibody [VPM65]

anti-CD14 antibody [VPM65] for IHC-Frozen sections,ELISA,Flow cytometry,Immunoprecipitation and Sheep,Bovine,Goat

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody

Overview

Product Description Mouse Monoclonal antibody [VPM65] recognizes CD14
Mouse Anti Sheep CD14 antibody, clone VPM65 recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules.
Tested Reactivity Bov, Goat, Sheep
Tested Application ELISA, FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone VPM65
Isotype IgG1
Target Name CD14
Antigen Species Sheep
Immunogen Ovine macrophages
Conjugation Un-conjugated
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:50 - 1:250
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or cells or 100 µl whole blood
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281048 Bovine CD14

Swiss-port # Q95122 Bovine Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Calculated MW 40 kDa
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt]