ARG42346

anti-CD146 antibody [P1H12] (PE)

anti-CD146 antibody [P1H12] (PE) for Flow cytometry and Rabbit,Dog,Mouse,Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [P1H12] recognizes CD146
Tested Reactivity Hu, Ms, Dog, Rb
Species Does Not React With Rat
Tested Application FACS
Specificity The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.
Host Mouse
Clonality Monoclonal
Clone P1H12
Isotype IgG1
Target Name CD146
Antigen Species Human
Immunogen Cultured Human umbilical cells.
Conjugation PE
Alternate Names Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; Cell surface glycoprotein MUC18; Melanoma cell adhesion molecule; CD146; MUC18; CD antigen CD146; S-endo 1 endothelial-associated antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4162 Human MCAM

GeneID: 84004 Mouse MCAM

Swiss-port # P43121 Human Cell surface glycoprotein MUC18

Swiss-port # Q8R2Y2 Mouse Cell surface glycoprotein MUC18

Gene Symbol MCAM
Gene Full Name melanoma cell adhesion molecule
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 72 kDa