ARG55936

anti-CD147 antibody [8D6]

anti-CD147 antibody [8D6] for Functional study,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [8D6] recognizes CD147
Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone 8D6
Isotype IgG1
Target Name CD147
Antigen Species Human
Immunogen Human T cell line Molt-13.
Conjugation Un-conjugated
Alternate Names OK; Tumor cell-derived collagenase stimulatory factor; Leukocyte activation antigen M6; TCSF; Extracellular matrix metalloproteinase inducer; OK blood group antigen; CD147; Basigin; M6; EMMPRIN; Collagenase stimulatory factor; CD antigen CD147; 5F7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
FuncStAssay-dependent
ICC/IF1:100 - 1:200
IHC-P1 - 2 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 682 Human BSG

Swiss-port # P35613 Human Basigin

Gene Symbol BSG
Gene Full Name basigin (Ok blood group)
Background The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. [UniProt]
Cellular Localization Cell surface, cytoplasmic
Calculated MW 42 kDa
PTM N-glycosylated.