ARG41553

anti-CD147 antibody

anti-CD147 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CD147
Tested Reactivity Ms
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD147
Antigen Species Mouse
Immunogen Recombinant protein of Mouse CD147.
Conjugation Un-conjugated
Alternate Names OK; Tumor cell-derived collagenase stimulatory factor; Leukocyte activation antigen M6; TCSF; Extracellular matrix metalloproteinase inducer; OK blood group antigen; CD147; Basigin; M6; EMMPRIN; Collagenase stimulatory factor; CD antigen CD147; 5F7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50
IHC-P1:20 - 1:500
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control C2C12
Observed Size ~ 53 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12215 Mouse BSG

Swiss-port # P18572 Mouse Basigin

Gene Symbol BSG
Gene Full Name basigin (Ok blood group)
Background The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In spermatozoa, localized on the principal piece of caput and in the middle piece during transit in the corpus and cauda epididymides (By similarity). [UniProt]
Calculated MW 42 kDa
PTM N-glycosylated. [UniProt]