ARG23094

anti-CD147 antibody [OX-47] (FITC)

anti-CD147 antibody [OX-47] (FITC) for Flow cytometry and Rat

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [OX-47] recognizes CD147
Mouse anti Rat CD147 antibody, clone OX-47 recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Paterson et al. 1987). CD147 is also present on some endothelia (Fossum et al. 1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossum et al. 1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Foda et al. 2001).
Tested Reactivity Rat
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone OX-47
Isotype IgG1
Target Name CD147
Antigen Species Rat
Immunogen T blasts from a mixed lymphocyte reaction.
Conjugation FITC
Alternate Names OK; Tumor cell-derived collagenase stimulatory factor; Leukocyte activation antigen M6; TCSF; Extracellular matrix metalloproteinase inducer; OK blood group antigen; CD147; Basigin; M6; EMMPRIN; Collagenase stimulatory factor; CD antigen CD147; 5F7

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:20
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25246 Rat BSG

Swiss-port # P26453 Rat Basigin

Gene Symbol Bsg
Gene Full Name basigin (Ok blood group)
Background The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. [UniProt]
Calculated MW 42 kDa
PTM N-glycosylated.