ARG42488

anti-CD15 antibody

anti-CD15 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes CD15
Tested Reactivity Hu, Rat
Tested Application IHC-P
Host Mouse
Clonality Monoclonal
Isotype IgG
Target Name CD15
Antigen Species Human
Immunogen Synthetic peptide of Human CD15.
Conjugation Un-conjugated
Alternate Names LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
Application Note IHC-P: Antigen Retrieval: 1) Heat mediated Sodium citrate buffer (pH 6.0) for 20 min, or 2) EDTA buffer (pH 8.0) at high pressure and temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Gene Symbol FUT4
Gene Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Background The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Function May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
Cellular Localization Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt]
Calculated MW 59 kDa