ARG42486

anti-CD15 antibody [FUT4/1478R]

anti-CD15 antibody [FUT4/1478R] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Recombinant Rabbit Monoclonal antibody [FUT4/1478R] recognizes CD15
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P
Host Rabbit
Clonality Monoclonal
Clone FUT4/1478R
Isotype IgG, kappa
Target Name CD15
Antigen Species Human
Immunogen Purified Human neutrophils.
Conjugation Un-conjugated
Alternate Names LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14345 Mouse FUT4

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Swiss-port # Q11127 Mouse Alpha-(1,3)-fucosyltransferase 4

Gene Symbol FUT4
Gene Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Background The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Function May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
Cellular Localization Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt]
Calculated MW 59 kDa