ARG66230

anti-CD15 antibody [MMA]

anti-CD15 antibody [MMA] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description

Mouse Monoclonal antibody [MMA] recognizes CD15

Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Specificity This antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody is a superior reagent for identifying of Hodgkin's lymphoma.
Host Mouse
Clonality Monoclonal
Clone MMA
Isotype IgM
Target Name CD15
Antigen Species Human
Immunogen U937 histiocytic lymphoma cells.
Conjugation Un-conjugated
Alternate Names LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IHC-P10 µg/ml
Application Note IHC-P: Antigen retrieval: Heat mediated.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by precipitation and chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2526 Human FUT4

Swiss-port # P22083 Human Alpha-(1,3)-fucosyltransferase 4

Gene Symbol FUT4
Gene Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Background The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Function May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt]
Highlight Related products:
CD15 antibodies; CD15 ELISA Kits; CD15 Duos / Panels; Anti-Mouse IgM secondary antibodies;
Related news:
Lymphoma
Calculated MW 59 kDa