ARG23612
anti-CD150 / SLAM antibody [9D1]
anti-CD150 / SLAM antibody [9D1] for Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [9D1] recognizes CD150 / SLAM. This product recognizes murine CD150, also known as signalling lymphocyte activation molecule (SLAM). Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and is upregulated on activated T-cells and stimulated macrophages. The extracellular domain of CD150 is the receptor for the measles virus (Erlenhoefer et al. 2001) and acts as a co-activator on T-cells and B-cells.Rat anti Mouse CD150 antibody, clone 9D1 has functional activity (Howie et al. 2002). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 9D1 |
| Isotype | IgG1 |
| Target Name | CD150 / SLAM |
| Antigen Species | Mouse |
| Immunogen | CHO cells stably transfected with Mouse CD150. |
| Conjugation | Un-conjugated |
| Alternate Names | Signaling lymphocytic activation molecule; IPO-3; CD150; SLAM; CDw150; CD antigen CD150 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QUM4 Mouse Signaling lymphocytic activation molecule |
|---|---|
| Gene Symbol | SLAMF1 |
| Gene Full Name | signaling lymphocytic activation molecule family member 1 |
| Function | High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. [UniProt] |
| Calculated MW | 37 kDa |
| PTM | Phosphorylated on tyrosine residues by FYN. [UniProt] |
