ARG22066

anti-CD152 / CTLA4 antibody [9H10] (low endotoxin)

anti-CD152 / CTLA4 antibody [9H10] (low endotoxin) for ELISA,Flow cytometry,Immunoprecipitation,Blocking and Mouse

Overview

Product Description Azide free and low endotoxin Syrian Hamster Monoclonal antibody [9H10] recognizes CD152 / CTLA4
Tested Reactivity Ms
Tested Application BL, ELISA, FACS, IP
Specificity Mouse CD152
Host Hamster
Clonality Monoclonal
Clone 9H10
Isotype IgG2
Target Name CD152 / CTLA4
Antigen Species Mouse
Immunogen Heat-killed Staphylococcus A bacteria coated with Mouse CTLA-4/Human IgG1 fusion protein
Conjugation Un-conjugated
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISA< 1 µg/ml
FACSAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12477 Mouse CTLA4

Swiss-port # P09793 Mouse Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Calculated MW 25 kDa
PTM N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.