ARG65419

anti-CD152 / CTLA4 antibody [BNI3]

anti-CD152 / CTLA4 antibody [BNI3] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IP
Specificity The clone BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
Host Mouse
Clonality Monoclonal
Clone BNI3
Isotype IgG2a
Target Name CD152 / CTLA4
Antigen Species Human
Immunogen Human CD152-IgG heavy chain fusion protein
Conjugation Un-conjugated
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 5 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Intracellular staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1493 Human CTLA4

Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

Clone References

Role of CTLA4 in the proliferation and survival of chronic lymphocytic leukemia.

WB / Human

Mittal AK et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Expanding the psoriasis disease profile: interrogation of the skin and serum of patients with moderate-to-severe psoriasis.

IHC-Fr / Human

Suárez-Fariñas M et al.
J Invest Dermatol.,  (2012)

publication_link

 

hr_line

A screen for endocytic motifs.

Kozik P et al.
Traffic.,  (2010)

publication_link

 

hr_line