ARG54228

anti-CD152 / CTLA4 antibody [BNI3] (PE)

anti-CD152 / CTLA4 antibody [BNI3] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [BNI3] recognizes CD152 / CTLA4
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody BNI3 recognizes human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
Host Mouse
Clonality Monoclonal
Clone BNI3
Isotype IgG2a
Target Name CD152 / CTLA4
Antigen Species Human
Immunogen Human CD152-IgG heavy chain fusion protein
Conjugation PE
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1493 Human CTLA4

Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 25 kDa
PTM N-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.

Clone References

Role of CTLA4 in the proliferation and survival of chronic lymphocytic leukemia.

WB / Human

Mittal AK et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Expanding the psoriasis disease profile: interrogation of the skin and serum of patients with moderate-to-severe psoriasis.

IHC-Fr / Human

Suárez-Fariñas M et al.
J Invest Dermatol.,  (2012)

publication_link

 

hr_line

A screen for endocytic motifs.

Kozik P et al.
Traffic.,  (2010)

publication_link

 

hr_line