ARG41604

anti-CD154 / CD40L antibody [24-31] (low endotoxin)

anti-CD154 / CD40L antibody [24-31] (low endotoxin) for Flow cytometry,Functional study,Immunocytochemistry and Human,Primates

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [24-31] recognizes CD154 / CD40L
Tested Reactivity Hu, NHuPrm
Tested Application FACS, FuncSt, ICC
Host Mouse
Clonality Monoclonal
Clone 24-31
Isotype IgG1
Target Name CD154 / CD40L
Antigen Species Human
Immunogen Human CD154 fusion protein.
Conjugation Un-conjugated
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 6 µg/ml
FuncStAssay-dependent
ICCAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 959 Human CD40LG

Swiss-port # P29965 Human CD40 ligand

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt]
Calculated MW 29 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.

N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.

Not O-glycosylated. [UniProt]