ARG65514

anti-CD154 / CD40L antibody [MR-1]

anti-CD154 / CD40L antibody [MR-1] for Flow cytometry,Functional study,ICC/IF,Immunohistochemistry,Immunoprecipitation,CyTOF®-candidate and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description

Hamster Monoclonal antibody [MR-1] recognizes CD154 / CD40L

Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC, IP
Specificity The clone MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Host Hamster
Clonality Monoclonal
Clone MR-1
Isotype IgG1
Target Name CD154 / CD40L
Antigen Species Mouse
Immunogen Activated mouse Th1 clone D1.6
Conjugation Un-conjugated
Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHCAssay-dependent
IPAssay-dependent
Application Note Functional studies: Blocking T cell costimulation in vivo.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: 6-8 hours activated mouse splenocytes.

Properties

Form Liquid
Purification Purified by protein A
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21947 Mouse CD40LG

Swiss-port # P27548 Mouse CD40 ligand

Gene Symbol Cd40lg
Gene Full Name CD40 ligand
Background CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Function Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity). [UniProt]
Highlight Related products:
CD154 antibodies; CD154 ELISA Kits; Anti-Hamster IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 29 kDa
PTM The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Clone References

Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction.

Lode HN et al.
J Clin Invest.,  (2000)

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Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L.

Hwang Yi et al.
Infect Immun.,  (2000)

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Functional CD40 ligand is expressed on epidermal Langerhans cells.

Salgado CG et al.
J Leukoc Biol.,  (1999)

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Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis.

Howard LM et al.
J Clin Invest.,  (1999)

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A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.

Noelle RJ et al.
Proc Natl Acad Sci U S A.,  (1992)

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