ARG65514
anti-CD154 / CD40L antibody [MR-1]
anti-CD154 / CD40L antibody [MR-1] for Flow cytometry,Functional study,ICC/IF,Immunohistochemistry,Immunoprecipitation,CyTOF®-candidate and Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Hamster Monoclonal antibody [MR-1] recognizes CD154 / CD40L |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC, IP |
| Specificity | The clone MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes. |
| Host | Hamster |
| Clonality | Monoclonal |
| Clone | MR-1 |
| Isotype | IgG1 |
| Target Name | CD154 / CD40L |
| Antigen Species | Mouse |
| Immunogen | Activated mouse Th1 clone D1.6 |
| Conjugation | Un-conjugated |
| Alternate Names | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Blocking T cell costimulation in vivo. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: 6-8 hours activated mouse splenocytes. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Cd40lg |
| Gene Full Name | CD40 ligand |
| Background | CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center. |
| Function | Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity). [UniProt] |
| Highlight | Related products: CD154 antibodies; CD154 ELISA Kits; Anti-Hamster IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 29 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
Clone References
Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction.
Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L.
Functional CD40 ligand is expressed on epidermal Langerhans cells.
Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis.
A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.
