ARG43314

anti-CD155 / Poliovirus Receptor antibody

anti-CD155 / Poliovirus Receptor antibody for Western blot,Immunoprecipitation and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CD155 / Poliovirus Receptor
Tested Reactivity Hu
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD155 / Poliovirus Receptor
Antigen Species Human
Immunogen Synthetic peptide derived from Human CD155 / Poliovirus Receptor.
Conjugation Un-conjugated
Alternate Names NECL5; CD antigen CD155; Nectin-like protein 5; PVS; Necl-5; HVED; Poliovirus receptor; TAGE4; NECL-5; CD155

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:40
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U87-MG
Observed Size 65, 70 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5817 Human PVR

Swiss-port # P15151 Human Poliovirus receptor

Gene Symbol PVR
Gene Full Name poliovirus receptor
Background The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Function Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.

(Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.

(Microbial infection) Acts as a receptor for Pseudorabies virus.

(Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells. [UniProt]
Cellular Localization Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Beta: Secreted. Isoform Gamma: Secreted. [UniProt]
Calculated MW 45 kDa
PTM N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4.

Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation. [UniProt]