ARG65543

anti-CD157 / BST1 antibody [SY11B5]

anti-CD157 / BST1 antibody [SY11B5] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Primates

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [SY11B5] recognizes CD157 / BST1
Tested Reactivity Hu, NHuPrm
Tested Application FACS, IHC-Fr, IP, WB
Specificity The mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPIanchored protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
Host Mouse
Clonality Monoclonal
Clone SY11B5
Isotype IgG1
Target Name CD157 / BST1
Antigen Species Human
Immunogen Human CD157
Conjugation Un-conjugated
Alternate Names cADPr hydrolase 2; ADP-ribosyl cyclase 2; Cyclic ADP-ribose hydrolase 2; BST-1; Bone marrow stromal antigen 1; CD157; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; CD antigen CD157; EC 3.2.2.6

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 12 µg/ml
IHC-Fr5 - 10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 683 Human BST1

Swiss-port # Q10588 Human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2

Gene Symbol BST1
Gene Full Name bone marrow stromal cell antigen 1
Background CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.
Function Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 36 kDa