ARG62738

anti-CD158d / KIR2DL4 antibody [mAb#33]

anti-CD158d / KIR2DL4 antibody [mAb#33] for Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [mAb#33] recognizes CD158d / KIR2DL4

Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IP, WB
Specificity The clone mAb 33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Host Mouse
Clonality Monoclonal
Clone mAb#33
Isotype IgG1
Target Name CD158d / KIR2DL4
Immunogen NK3.3 cells and KIR2DL4-Ig fusion protein
Conjugation Un-conjugated
Alternate Names KIR103; CD158 antigen-like family member D; Killer cell immunoglobulin-like receptor 2DL4; CD158D; CD antigen CD158d; G9P; Killer cell inhibitory receptor 103AS; KIR-103AS; KIR103AS; MHC class I NK cell receptor KIR103AS

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 6 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: Cytokine secretion studies.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3805 Human KIR2DL4

Swiss-port # Q99706 Human Killer cell immunoglobulin-like receptor 2DL4

Gene Symbol KIR2DL4
Gene Full Name killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
Background CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
Function Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. [UniProt]
Research Area Immune System antibody
Calculated MW 41 kDa

Clone References

Soluble human leukocyte antigen-g5 activates extracellular signal-regulated protein kinase signaling and stimulates trophoblast invasion.

WB / Human

Guo Y et al.
PLoS One.,  (2013)

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Activation of NK cells by an endocytosed receptor for soluble HLA-G.

Rajagopalan S et al.
PLoS Biol.,  (2006)

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HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells.

LeMaoult J et al.
FASEB J.,  (2005)

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KIR2DL4 (CD158d) genotype influences expression and function in NK cells.

Goodridge JP et al.
J Immunol.,  (2003)

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Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells.

Rajagopalan S et al.
J Immunol.,  (2001)

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