ARG42255

anti-CD158f / KIR2DL5 antibody [UP-R1]

anti-CD158f / KIR2DL5 antibody [UP-R1] for Flow cytometry,ICC/IF and Human

Overview

Product Description Mouse Monoclonal antibody [UP-R1] recognizes CD158f / KIR2DL5
Tested Reactivity Hu
Tested Application FACS, ICC/IF
Specificity The mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.
Host Mouse
Clonality Monoclonal
Clone UP-R1
Isotype IgG1, kappa
Target Name CD158f / KIR2DL5
Antigen Species Human
Immunogen Human CD158f-Ig fusion protein.
Conjugation Un-conjugated
Alternate Names CD antigen CD158f1; KIR2DL5.3; KIR2DL5.1; Killer cell immunoglobulin-like receptor 2DL5A; KIR2DL5; CD158F

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 57292 Human KIR2DL5A

Swiss-port # Q8N109 Human Killer cell immunoglobulin-like receptor 2DL5A

Gene Symbol KIR2DL5A
Gene Full Name killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A
Background Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]
Function Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 41 kDa