ARG54305
anti-CD158f / KIR2DL5 antibody [UP-R1] (PE)
anti-CD158f / KIR2DL5 antibody [UP-R1] (PE) for Flow cytometry and Human
Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [UP-R1] recognizes CD158f |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody UPR1 recognizes CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | UP-R1 |
| Isotype | IgG1 |
| Target Name | CD158f / KIR2DL5 |
| Antigen Species | Human |
| Immunogen | Human CD158f-Ig fusion protein |
| Conjugation | PE |
| Alternate Names | CD antigen CD158f1; KIR2DL5.3; KIR2DL5.1; Killer cell immunoglobulin-like receptor 2DL5A; KIR2DL5; CD158F |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8N109 Human Killer cell immunoglobulin-like receptor 2DL5A |
|---|---|
| Gene Symbol | KIR2DL5A |
| Gene Full Name | killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A |
| Background | CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population. |
| Function | Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 41 kDa |
