ARG40693

anti-CD16 antibody

anti-CD16 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD16
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD16
Antigen Species Human
Immunogen Recombinant protein corresponding to Q101-D166 of Human CD16.
Conjugation Un-conjugated
Alternate Names FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2214 Human FCGR3A

Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A

Gene Symbol FCGR3A
Gene Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Background This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Secreted. Note=Exists also as a soluble receptor. [UniProt]
Highlight Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 29 kDa
PTM Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.

The soluble form is produced by a proteolytic cleavage. [UniProt]