ARG62743

anti-CD16 antibody [MEM-154] (FITC)

anti-CD16 antibody [MEM-154] (FITC) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description

FITC-conjugated Mouse Monoclonal antibody [MEM-154] recognizes CD16

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-154 reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). MEM-154 reacts with CD16+ granulocytes.
HLDA V; WS Code M MA068
HLDA V; WS Code NK NK51
Host Mouse
Clonality Monoclonal
Clone MEM-154
Isotype IgG1
Target Name CD16
Antigen Species Human
Immunogen Human granulocytes
Conjugation FITC
Alternate Names FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2214 Human FCGR3A

Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A

Gene Symbol FCGR3A
Gene Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Function Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
Highlight Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 29 kDa
PTM Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage.

Clone References

Fc gamma receptor 3A polymorphism and risk for HIV-associated cryptococcal disease.

Rohatgi S et al.
MBio.,  (2013)

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IgG4 subclass antibodies impair antitumor immunity in melanoma.

IHC-Fr / Human

Karagiannis P et al.
J Clin Invest.,  (2013)

publication_link

 

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Morelloflavone, a biflavonoid inhibitor of migration-related kinases, ameliorates atherosclerosis in mice.

Pinkaew D et al.
Am J Physiol Heart Circ Physiol.,  (2012)

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Expression of human FcgammaRIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding.

FACS / Human

Armour KL et al.
J Immunol Methods.,  (2010)

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The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.

Tamm A et al.
J Immunol.,  (1996)

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