ARG62741

anti-CD16 antibody [MEM-168]

anti-CD16 antibody [MEM-168] for Flow cytometry and Human,Primates,Pig

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-168] recognizes CD16
Tested Reactivity Hu, NHuPrm, Pig
Tested Application FACS
Specificity The clone MEM-168 reacts with CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).
Host Mouse
Clonality Monoclonal
Clone MEM-168
Isotype IgM
Target Name CD16
Antigen Species Human
Immunogen Human granulocytes
Conjugation Un-conjugated
Alternate Names FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by gel filtration and precipitation methods.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2214 Human FCGR3A

Swiss-port # P08637 Human Low affinity immunoglobulin gamma Fc region receptor III-A

Gene Symbol FCGR3A
Gene Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Function Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
Highlight Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Mouse IgM secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
Calculated MW 29 kDa
PTM Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage.