ARG54259
anti-CD161 antibody [HP-3G10] (PE)
anti-CD161 antibody [HP-3G10] (PE) for Flow cytometry and Human,Primates
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [HP-3G10] recognizes CD161 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone HP-3G10 recognizes CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HP-3G10 |
| Isotype | IgG1 |
| Target Name | CD161 |
| Antigen Species | Human |
| Immunogen | human NK cells |
| Conjugation | PE |
| Alternate Names | CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12918 Human Killer cell lectin-like receptor subfamily B member 1 |
|---|---|
| Gene Symbol | KLRB1 |
| Gene Full Name | killer cell lectin-like receptor subfamily B, member 1 |
| Background | CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on a majority of NK cells, NKT cells, and e.g. Th17 cells and CD3+ thymocytes. Although rat CD161 has three isoforms (a, b, c), the human CD161 is expressed as one isoform. |
| Function | Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 25 kDa |
| PTM | N-glycosylated. Contains sialic acid residues. |
Clone References
CD161 (human NKR-P1A) signaling in NK cells involves the activation of acid sphingomyelinase.
