ARG22501

anti-CD161 antibody [PK136]

anti-CD161 antibody [PK136] for Flow cytometry,Immunoprecipitation,CyTOF®-candidate and Mouse

Overview

Product Description Mouse Monoclonal antibody [PK136] recognizes CD161
This antibody recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.
Tested Reactivity Ms
Species Does Not React With Hu, Rat
Tested Application CyTOF®-candidate, FACS, IP
Host Mouse
Clonality Monoclonal
Clone PK136
Isotype IgG2a
Target Name CD161
Antigen Species Mouse
Immunogen Spleen and bone marrow cells from CE mice.
Conjugation Un-conjugated
Alternate Names CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACSNeat
IPAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100043861 Mouse KLRB1

Swiss-port # Q0ZUP1 Mouse Killer cell lectin-like receptor subfamily B member 1

Gene Symbol Klrb1b
Gene Full Name killer cell lectin-like receptor subfamily B member 1B
Background Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]
Function Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt]
Highlight Related products:
CD161 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Calculated MW 25 kDa
PTM N-glycosylated. Contains sialic acid residues.