ARG23181
anti-CD16.2 / FcgRIV antibody [AT137]
anti-CD16.2 / FcgRIV antibody [AT137] for IHC-Frozen sections,Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [AT137] recognizes CD16.2 / FcgRIV Mouse anti human CD16-2 antibody, clone AT137, recognizes CD16-2, also known as FcRIV. CD16-2 is a low affinity activatory receptor that binds to IgG2a and IgG2b with intermediate affinity. FcRIV has no affinity for IgG1 or IgG3 and therefore has a unique subclass specificity when compared to other Fc receptors. Expression of CD16-2 is restricted to cells of the myeloid lineage. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | AT137 |
| Isotype | IgG2a |
| Target Name | CD16.2 / FcgRIV |
| Antigen Species | Mouse |
| Immunogen | Fusion Protein consisting of mouse CD16-2 and rat CD4. |
| Conjugation | Un-conjugated |
| Alternate Names | Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | TRIS buffered saline and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Fcgr4 |
|---|---|
| Gene Full Name | Fc receptor, IgG, low affinity IV |
| Function | Receptor for the Fc region of immunoglobulin gamma (PubMed:16039578). Also acts as a receptor for the Fc region of immunoglobulin epsilon (PubMed:17558411, PubMed:18949059). Binds with intermediate affinity to both IgG2a and IgG2b (PubMed:16039578, PubMed:17558411, PubMed:19795417). Can bind to IgG2a and IgG2b monomers (PubMed:18949059). Does not display binding to IgG1 or IgG3 (PubMed:16039578). Mediates neutrophil activation by IgG complexes redundantly with Fcgr3 (PubMed:18097064). Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a (PubMed:25824719). Binds with low affinity to both the a and b allotypes of IgE (PubMed:18949059). Has also been shown to bind to IgE allotype a only but not to allotype b (PubMed:17558411). Binds aggregated IgE but not the monomeric form and bound monomeric IgG is readily displaced by IgE complexes (PubMed:18949059). Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions (PubMed:17558411, PubMed:18949059). [UniProt] |
| Calculated MW | 28 kDa |
