ARG22856

anti-CD163 antibody [2A10/11]

anti-CD163 antibody [2A10/11] for IHC-Frozen sections,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Pig

M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody

Overview

Product Description Mouse Monoclonal antibody [2A10/11] recognizes CD163
Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a 120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchez et al. 1999). Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003).
Tested Reactivity Pig
Tested Application FACS, ICC/IF, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone 2A10/11
Isotype IgG1
Target Name CD163
Antigen Species Pig
Immunogen Porcine alveolar macrophages.
Conjugation Un-conjugated
Alternate Names sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Clone 2A10/11 detects a band of ~ 120 kD in alveolar macrophage cell lysates under non-reducing conditions. Clone 2A10/11 recognizes porcine CD163 under non-reducing conditions.
FACS: Use 10 µl of the suggested working dilution to 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 397031 Pig CD163

Swiss-port # Q2VL90 Pig Scavenger receptor cysteine-rich type 1 protein M130

Gene Symbol CD163
Gene Full Name CD163 molecule
Background CD163 protein is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Function CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt]
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Research Area M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
Calculated MW 125 kDa
PTM A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.