ARG42302

anti-CD169 / Siglec 1 antibody [7-239] (APC)

anti-CD169 / Siglec 1 antibody [7-239] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [7-239] recognizes CD169 / Siglec 1
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.
Host Mouse
Clonality Monoclonal
Clone 7-239
Isotype IgG1
Target Name CD169 / Siglec 1
Antigen Species Human
Immunogen Human rhinovirus 14-infected monocyte-derived dendritic cells.
Conjugation APC
Alternate Names CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6614 Human SIGLEC1

Swiss-port # Q9BZZ2 Human Sialoadhesin

Gene Symbol SIGLEC1
Gene Full Name sialic acid binding Ig-like lectin 1, sialoadhesin
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Calculated MW 183 kDa