ARG23465

anti-CD169 / Siglec 1 antibody [ED3]

anti-CD169 / Siglec 1 antibody [ED3] for IHC-Frozen sections,Flow cytometry,ICC/IF,Immunoprecipitation and Rat

Overview

Product Description Mouse Monoclonal antibody [ED3] recognizes CD169 / Siglec 1
Tested Reactivity Rat
Tested Application FACS, ICC/IF, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone ED3
Isotype IgG2a
Target Name CD169 / Siglec 1
Antigen Species Rat
Immunogen Rat Spleen cell homogenate
Conjugation Un-conjugated
Alternate Names CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:500
ICC/IFAssay-dependent
IHC-Fr1:50 - 1:250
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol SIGLEC1
Gene Full Name sialic acid binding Ig-like lectin 1, sialoadhesin
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt]
Calculated MW 183 kDa