ARG42427

anti-CD170 / Siglec 5 antibody [1A5]

anti-CD170 / Siglec 5 antibody [1A5] for Flow cytometry,Immunoprecipitation,IHC-Frozen sections,ICC/IF,ELISA and Human,Primates

Overview

Product Description Mouse Monoclonal antibody [1A5] recognizes CD170 / Siglec 5
Tested Reactivity Hu, NHuPrm
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IP
Specificity The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Host Mouse
Clonality Monoclonal
Clone 1A5
Isotype IgG1
Target Name CD170 / Siglec 5
Antigen Species Human
Immunogen Fusion protein composed of Human CD170 extracellular domain and Fc region of Human IgG1.
Conjugation Un-conjugated
Alternate Names OBBP2; OB-binding protein 2; CD170; Sialic acid-binding Ig-like lectin 5; SIGLEC-5; CD antigen CD170; CD33 antigen-like 2; OB-BP2; CD33L2; Siglec-5; Obesity-binding protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IHC-Fr5 - 10 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8778 Human SIGLEC5

Swiss-port # O15389 Human Sialic acid-binding Ig-like lectin 5

Gene Symbol SIGLEC5
Gene Full Name sialic acid binding Ig-like lectin 5
Background This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 61 kDa