ARG42427
anti-CD170 / Siglec 5 antibody [1A5]
anti-CD170 / Siglec 5 antibody [1A5] for Flow cytometry,Immunoprecipitation,IHC-Frozen sections,ICC/IF,ELISA and Human,Primates
Overview
| Product Description | Mouse Monoclonal antibody [1A5] recognizes CD170 / Siglec 5 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IP |
| Specificity | The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A5 |
| Isotype | IgG1 |
| Target Name | CD170 / Siglec 5 |
| Antigen Species | Human |
| Immunogen | Fusion protein composed of Human CD170 extracellular domain and Fc region of Human IgG1. |
| Conjugation | Un-conjugated |
| Alternate Names | OBBP2; OB-binding protein 2; CD170; Sialic acid-binding Ig-like lectin 5; SIGLEC-5; CD antigen CD170; CD33 antigen-like 2; OB-BP2; CD33L2; Siglec-5; Obesity-binding protein 2 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15389 Human Sialic acid-binding Ig-like lectin 5 |
|---|---|
| Gene Symbol | SIGLEC5 |
| Gene Full Name | sialic acid binding Ig-like lectin 5 |
| Background | This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012] |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 61 kDa |
