ARG54417
anti-CD172a / SIRP alpha antibody
anti-CD172a / SIRP alpha antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Neuroscience antibody; Cardiomyocyte Cell Surface Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD172a / SIRP alpha |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Bov |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD172a / SIRP alpha |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to the C-terminus of human SIRPα. |
| Conjugation | Un-conjugated |
| Alternate Names | CD172A; p84; SHPS1; SHPS-1; CD172 antigen-like family member A; Sirp-alpha-3; Sirp-alpha-1; BIT; MYD-1; MFR; Bit; PTPNS1; CD antigen CD172a; Inhibitory receptor SHPS-1; SIRP; MyD-1 antigen; Sirp-alpha-2; Tyrosine-protein phosphatase non-receptor type substrate 1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Macrophage fusion receptor; Brain Ig-like molecule with tyrosine-based activation motifs; P84; SHP substrate 1 |
Application Instructions
| Cross Reactivity Note | This antibody recognizes full-length human SIRPα (75-110 kD). The sequence of this peptide differs from those of mouse, rat, and bovine SIRPα by one amino acid, so this antibody may be used to detect SIRPα of these species. | ||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Whole cell lysate from THP-1 cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by immunoaffinity chroma-tography. |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P78324 Human Tyrosine-protein phosphatase non-receptor type substrate 1 |
|---|---|
| Gene Symbol | SIRPA |
| Gene Full Name | signal-regulatory protein alpha |
| Background | Protein tyrosine phosphatases (PTPases), such as SHP-1 and SHP-2, are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse, and cattle by several laboratories and was designated SIRPα, SHPS-1, and MyD-1. SIRPα/SHPS-1 is a new gene family containing at least fifteen members. SIRPα/SHPS-1 is a substrate of manyactivated tyrosine kinases such as insulin receptor, EGFR, PDGFR, and src, and is a specific docking protein for SHP-2. SIRPα/SHPS-1 has regulatory effects on cellular responses induced by serum,growth factors, insulin, oncogenes, and cell adhesion and plays a general role in different physiological and pathological processes. |
| Function | Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30142 Cardiomyocyte Cell Surface Marker Antibody Panel (Cx43, Desmin, SIRP) Related products: CD172a antibodies; CD172a Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Cardiomyocyte Cell Surface Marker antibody |
| Calculated MW | 55 kDa |
| PTM | N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. [UniProt] |
