ARG66075

anti-CD178 / Fas Ligand antibody

anti-CD178 / Fas Ligand antibody for ELISA,Western blot,Neutralizing,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Goat Polyclonal antibody recognizes CD178 / Fas Ligand
Tested Reactivity Hu, Ms
Tested Application ELISA, IHC-P, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CD178 / Fas Ligand
Antigen Species Human
Immunogen CHO cells derived recombinant Human Fas Ligand.
Conjugation Un-conjugated
Alternate Names FasL ICD; SPPL2A-processed FasL form; Apoptosis antigen ligand; CD95 ligand; CD178; Fas antigen ligand; CD95-L; Receptor-binding FasL ectodomain; FasL; SPA; TNFSF6; CD95L; FASL; Fas ligand; APTL; APT1LG1; ALPS1B; sFasL; Soluble Fas ligand; Tumor necrosis factor ligand superfamily member 6; APL; CD antigen CD178

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66076 as a detection antibody
IHC-P0.75 - 2.5 µg/ml
Neut0.08 - 0.1 µg/ml (To yield [ND50] of the biological activity of sFasL/Apo1L (10 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 356 Human FASLG

Swiss-port # P48023 Human Tumor necrosis factor ligand superfamily member 6

Gene Symbol FASLG
Gene Full Name Fas ligand (TNF superfamily, member 6)
Background This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt]
Calculated MW 31 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Monoubiquitinated.