ARG20847
anti-CD18 / LFA1 beta antibody [C71/16] (Biotin)
anti-CD18 / LFA1 beta antibody [C71/16] (Biotin) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,ELISA and Mouse
Overview
| Product Description | Biotin-conjugated Rat Monoclonal antibody [C71/16] recognizes CD18 / LFA1 beta |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Mouse CD18. The clone C71/16 has not been reported to inhibit cell adhesion. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | C71/16 |
| Isotype | IgG2a, kappa |
| Target Name | CD18 / LFA1 beta |
| Antigen Species | Mouse |
| Immunogen | Cell membrane glycoproteins derived from mouse BW5147 T cell lymphoma cells |
| Conjugation | Biotin |
| Alternate Names | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGB2 |
| Gene Full Name | integrin beta 2 |
| Background | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
