ARG65487

anti-CD18 / LFA1 beta antibody [MEM-148] (low endotoxin)

anti-CD18 / LFA1 beta antibody [MEM-148] (low endotoxin) for Flow cytometry,Immunoprecipitation,Western blot,Functional study and Human

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [MEM-148] recognizes CD18 / LFA1 beta
Tested Reactivity Hu
Species Does Not React With Pig
Tested Application FACS, FuncSt, IP, WB
Specificity The clone MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
HLDA VI; WS Code AS A052
Host Mouse
Clonality Monoclonal
Clone MEM-148
Isotype IgG1
Target Name CD18 / LFA1 beta
Immunogen Peripheral blood mononuclear cells
Conjugation Un-conjugated
Alternate Names MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS8 µg/ml
FuncStAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: The clone MEM-148 is excellent marker of activated myeloid cells.
WB: Under non-reducing condition.
Functional studies: The clone MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3689 Human ITGB2

Swiss-port # P05107 Human Integrin beta-2

Gene Symbol ITGB2
Gene Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 85 kDa
PTM Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

Clone References

LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.

BL / Human

Ghislin S et al.
BMC Cancer.,  (2012)

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The context of HLA-DR/CD18 complex in the plasma membrane governs HLA-DR-derived signals in activated monocytes.

Doisne JM et al.
Mol Immunol.,  (2008)

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Indirubin, a Chinese anti-leukaemia drug, promotes neutrophilic differentiation of human myelocytic leukaemia HL-60 cells.

WB / Human

Suzuki K et al.
Br J Haematol.,  (2005)

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A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.

Drbal K et al.
Blood.,  (2001)

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A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins.

Drbal K et al.
Immunobiology.,  (2001)

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