ARG62750

anti-CD18 / LFA1 beta antibody [MEM-48] (Biotin)

anti-CD18 / LFA1 beta antibody [MEM-48] (Biotin) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [MEM-48] recognizes CD18 / LFA1 beta
Tested Reactivity Hu
Species Does Not React With Dog
Tested Application FACS
Specificity The clone MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Host Mouse
Clonality Monoclonal
Clone MEM-48
Isotype IgG1
Target Name CD18 / LFA1 beta
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Conjugation Biotin
Alternate Names MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3689 Human ITGB2

Swiss-port # P05107 Human Integrin beta-2

Gene Symbol ITGB2
Gene Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 85 kDa
PTM Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

Clone References

Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.

ICC/IF / 

Giese S et al.
PLoS Pathog.,  (2014)

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Analysis on the relevance of asthma susceptibility with the alteration of integrin β 4 expression.

WB / Human

Xiang Y et al.
PLoS One.,  (2014)

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Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow.

Evani SJ et al.
FASEB J.,  (2013)

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NKp80 defines and stimulates a reactive subset of CD8 T cells.

Kuttruff S et al.
Blood.,  (2009)

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Human leukocytes contain a large pool of free forms of CD18.

Drbal K et al.
Biochem Biophys Res Commun.,  (2000)

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