ARG23052

anti-CD18 / LFA1 beta antibody [YFC118.3] (low endotoxin)

anti-CD18 / LFA1 beta antibody [YFC118.3] (low endotoxin) for IHC-Frozen sections,Flow cytometry,Functional study,Immunoprecipitation and Human,Dog,Guinea pig

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [YFC118.3] recognizes CD18 / LFA1 beta
Tested Reactivity Hu, Dog, Gpig
Tested Application FACS, FuncSt, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone YFC118.3
Isotype IgG2b
Target Name CD18 / LFA1 beta
Antigen Species Human
Immunogen Human neutrophils.
Conjugation Un-conjugated
Alternate Names MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3689 Human ITGB2

Swiss-port # P05107 Human Integrin beta-2

Gene Symbol ITGB2
Gene Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Background This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt]
Calculated MW 85 kDa
PTM Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).