ARG65445
anti-CD180 / RP105 antibody [G28-8]
anti-CD180 / RP105 antibody [G28-8] for Flow cytometry,Functional study,Immunoprecipitation and Human
Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [G28-8] recognizes CD180 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IP |
| Specificity | The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | G28-8 |
| Isotype | IgG1 |
| Target Name | CD180 / RP105 |
| Antigen Species | Human |
| Immunogen | Human tonsillar B cells |
| Conjugation | Un-conjugated |
| Alternate Names | LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Induction of B cell proliferation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD180 |
| Gene Full Name | CD180 molecule |
| Background | CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size. |
| Function | May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt] |
| Research Area | Immune System antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 74 kDa |
Clone References
Caspase activity is required for stimulated B lymphocytes to enter the cell cycle.
Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation.
