ARG42261

anti-CD180 / RP105 antibody [G28-8] (low endotoxin)

anti-CD180 / RP105 antibody [G28-8] (low endotoxin) for Flow cytometry,Immunoprecipitation,Functional study and Human

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [G28-8] recognizes CD180 / RP105
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP
Specificity The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Host Mouse
Clonality Monoclonal
Clone G28-8
Isotype IgG1, kappa
Target Name CD180 / RP105
Antigen Species Human
Immunogen Human tonsillar B cells.
Conjugation Un-conjugated
Alternate Names LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 µg/ml
FuncStAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4064 Human CD180

Swiss-port # Q99467 Human CD180 antigen

Gene Symbol CD180
Gene Full Name CD180 molecule
Background CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]
Function May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 74 kDa