ARG23128

anti-CD19 antibody [LE-CD19]

anti-CD19 antibody [LE-CD19] for ELISA,Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody

Overview

Product Description Mouse Monoclonal antibody [LE-CD19] recognizes CD19
Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and prloliferation.B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004)Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.
Tested Reactivity Hu
Tested Application ELISA, FACS, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone LE-CD19
Isotype IgG1
Target Name CD19
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide around the C-terminus of Human CD19. (CGPDPAWGGGGRMGTWSTR)
Conjugation Un-conjugated
Alternate Names Differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD antigen CD19; CVID3

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:100 - 1:200
IHC-P1:100 - 1:200
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 930 Human CD19

Swiss-port # P15391 Human B-lymphocyte antigen CD19

Gene Symbol CD19
Gene Full Name CD19 molecule
Background CD19: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Function CD19 functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS)
ARG30307 Pre-B Cell Marker Antibody Panel (CD19, CD25, CD38, CD40, CD45) (FACS)
ARG30308 Immature B Cell Marker Antibody Panel (CD19, CD20, CD22, IgM Fc) (FACS)
ARG30313 General Lymphocyte Marker Antibody Panel (CD3, CD14, CD16, CD19, CD56)
Related products:
CD19 antibodies; CD19 ELISA Kits; CD19 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Lymphoma
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; B cell Marker antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody; Immature B Cell Marker antibody; Follicular dendritic cells antibody
Calculated MW 61 kDa
PTM Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. [UniProt]