ARG54318

anti-CD195 / CCR5 antibody

anti-CD195 / CCR5 antibody for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD195 / CCR5
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD195 / CCR5
Antigen Species Human
Immunogen Peptide corresponding to aa 6-20 of human CCR5.
Conjugation Un-conjugated
Alternate Names CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor

Application Instructions

Application Note Western blot: use at 1:1,000 - 1:2,000 dilution.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control THP-1

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1234 Human CCR5

Swiss-port # P51681 Human C-C chemokine receptor type 5

Gene Symbol CCR5
Gene Full Name chemokine (C-C motif) receptor 5 (gene/pseudogene)
Background Human immunodeficiency virus (HIV) and related viruses require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, Bonzo, GPR1, and V28, have been identified as HIV coreceptors. Among them, CCR5 is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains. CCR5 is required for infection by HIV-1, HIV-2, and SIV. The β-chemokines RANTES, MIP-1α, and MIP-1β are the ligands for CCR5 and prevent infection by macrophage-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. Messenger RNA for CCR5 is expressed in lymphoid cells and tissues.
Function Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 41 kDa
PTM Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.