ARG65544

anti-CD195 / CCR5 antibody [T21/8]

anti-CD195 / CCR5 antibody [T21/8] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [T21/8] recognizes CD195 / CCR5
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IP, WB
Specificity The mouse monoclonal antibody T21/8 recognizes the Nteminus of CD195, an approximately 45 kDa Gprotein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.
Host Mouse
Clonality Monoclonal
Clone T21/8
Isotype IgG1
Target Name CD195 / CCR5
Immunogen CCR5 peptide (Met1-Lys22) KLH conjugate
Conjugation Un-conjugated
Alternate Names CHEMR13; CD195; C-C chemokine receptor type 5; CKR-5; CCCKR5; CCR-5; CD antigen CD195; CKR5; CC-CKR-5; IDDM22; CCR5; CMKBR5; C-C CKR-5; HIV-1 fusion coreceptor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Do not boil cell lysates prior to SDS-PAGE.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1234 Human CCR5

Swiss-port # P51681 Human C-C chemokine receptor type 5

Gene Symbol CCR5
Gene Full Name chemokine (C-C motif) receptor 5 (gene/pseudogene)
Background CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.
Function Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 41 kDa
PTM Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.