ARG65392

anti-CD197 / CCR7 antibody [4B12]

anti-CD197 / CCR7 antibody [4B12] for CyTOF®-candidate,Flow cytometry,Immunoprecipitation and Mouse

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rat Monoclonal antibody [4B12] recognizes CD197 / CCR7
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, IP
Specificity The rat monoclonal antibody 4B12 recognizes murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa Gproteincoupled receptor for chemokines CCL19 and CCL21.
Host Rat
Clonality Monoclonal
Clone 4B12
Isotype IgG2a
Target Name CD197 / CCR7
Antigen Species Mouse
Immunogen Murine CD197-transfected RBL-2H3 cells
Conjugation Un-conjugated
Alternate Names C-C chemokine receptor type 7; CD197; CD antigen CD197; CCR-7; CC-CKR-7; BLR2; CMKBR7; C-C CKR-7; MIP-3 beta receptor; EBV-induced G-protein coupled receptor 1; EBI1; Epstein-Barr virus-induced G-protein coupled receptor 1; CDw197

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-G affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12775 Mouse CCR7

Swiss-port # P47774 Mouse C-C chemokine receptor type 7

Gene Symbol Ccr7
Gene Full Name chemokine (C-C motif) receptor 7
Background CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.
Function Receptor for the MIP-3-beta chemokine. [UniProt]
Highlight Related products:
CD197 antibodies; Anti-Rat IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 43 kDa

Clone References

The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells.

Waldner H et al.
J Immunol.,  (2006)

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CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions.

Ohl L et al.
Immunity.,  (2004)

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Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.

Ritter U et al.
J Leukoc Biol.,  (2004)

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