ARG55937

anti-CD1a antibody [O10]

anti-CD1a antibody [O10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [O10] recognizes CD1a
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone O10
Isotype IgG1, kappa
Target Name CD1a
Antigen Species Human
Immunogen Human thymus cells.
Conjugation Un-conjugated
Alternate Names R4; FCB6; CD antigen CD1a; T6; T-cell surface antigen T6/Leu-6; hTa1 thymocyte antigen; T-cell surface glycoprotein CD1a; CD1; HTA1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 5 µg/ml
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 909 Human CD1A

Swiss-port # P06126 Human T-cell surface glycoprotein CD1a

Gene Symbol CD1A
Gene Full Name CD1a molecule
Background This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Function Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. [UniProt]
Cellular Localization Cell surface
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Calculated MW 37 kDa