ARG23174

anti-CD1d antibody [WTH1]

anti-CD1d antibody [WTH1] for IHC-Frozen sections,ELISA,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Rat,Mouse

Overview

Product Description Mouse Monoclonal antibody [WTH1] recognizes CD1d
Mouse anti Rat CD1d antibody, clone WTH1 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.Clone WTH1 recognizes a different, non-overlapping epitope to clone WTH2 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2.Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.
Tested Reactivity Ms, Rat
Tested Application ELISA, FACS, FuncSt, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone WTH1
Isotype IgG2a
Target Name CD1d
Antigen Species Rat
Immunogen Rat CD1d-transduced M12.4.1.C3 cells.
Conjugation Un-conjugated
Alternate Names CD antigen CD1d; CD1A; R3G1; Antigen-presenting glycoprotein CD1d; R3

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:2000
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FuncSt: This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
WB: Clone WTH1 recognizes CD1d under non-reducing conditions.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Cd1d1
Gene Full Name CD1d1 antigen
Background This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. [UniProt]
Calculated MW 38 kDa