ARG53788

anti-CD2 antibody [MEM-65] (PE)

anti-CD2 antibody [MEM-65] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [MEM-65] recognizes CD2

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells; also expressed by all thymocytes.
HLDA VI; WS Code T 6T-012
Host Mouse
Clonality Monoclonal
Clone MEM-65
Isotype IgG1
Target Name CD2
Antigen Species Human
Immunogen Human peripheral T cells.
Conjugation PE
Alternate Names T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 914 Human CD2

Swiss-port # P06729 Human T-cell surface antigen CD2

Gene Symbol CD2
Gene Full Name CD2 molecule
Background CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Function CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 39 kDa

Clone References

New monoclonal antibodies to human leucocyte surface molecule CD2.

Drbal K et al.
Folia Biol (Praha).,  (1997)

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