ARG22933

anti-CD2 antibody [OX-34]

anti-CD2 antibody [OX-34] for IHC-Frozen sections,Flow cytometry,ICC/IF,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections and Rat

Overview

Product Description Mouse Monoclonal antibody [OX-34] recognizes CD2
Mouse anti Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williams et al. 1987).
Tested Reactivity Rat
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone OX-34
Isotype IgG2a
Target Name CD2
Antigen Species Rat
Immunogen Activated Rat T helper cells.
Conjugation Un-conjugated
Alternate Names T-cell surface antigen T11/Leu-5; LFA-3 receptor; T-cell surface antigen CD2; SRBC; Erythrocyte receptor; CD antigen CD2; T11; Rosette receptor; LFA-2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note IHC-P: We recommend the use of PLP fixation for optimal results.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 497761 Rat CD2

Swiss-port # P08921 Rat T-cell surface antigen CD2

Gene Symbol Cd2
Gene Full Name Cd2 molecule
Background CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]
Function CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. [UniProt]
Calculated MW 39 kDa